t2cure is focussing on bone marrow-derived cells (BMCs) for use in cardiovascular repair. Bone marrow mononuclear cells (BMCs) can easily be obtained by aspirating bone marrow from the iliac crest under local anaesthesia. BMCs contain a variety of stem cell and progenitor cells, primarily haematopoietic stem cells, mesenchymal stem cell and other multipotential adult progenitor cells.
The mononuclear cell fraction is purified from the bone marrow aspirate by Ficoll gradient centrifugation at a central GMP-compliant manufacturing facility. For cardiac diseases, BMCs are then injected into the coronary arteries via catheter without further in vitro cell propagation. The same principle is applied in the peripheral setting, where BMCs are injected via catheter in the arteries of the limbs.